Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXM1

Cell type

Cell type Class
Bone
Cell type
U2OS
Tissue
bone
Lineage
mesoderm
Description
osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Attributes by original data submitter

Sample

source_name
osteosarcoma
tissue
bone
cell line
U2OS
time point
9h
antibody
FoxM1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were lysed and chromatin was micrococcal treated. The fragmented chromatin was pre-cleared with Dynabeads. Gamma-tubulin-, PCNA-, Mcm5-, and FoxM1-DNA-complexes were isolated with the correspondant antibody and isolated with Dynabeads. ChIP DNA using the Smarter ThruPLEX DNA-seq Kit (cat# R400676, Takara) with HT dual indexes (cat# R400660, Takara)

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
8721667
Reads aligned (%)
77.4
Duplicates removed (%)
20.1
Number of peaks
178 (qval < 1E-05)

hg19

Number of total reads
8721667
Reads aligned (%)
76.0
Duplicates removed (%)
20.7
Number of peaks
145 (qval < 1E-05)

Base call quality data from DBCLS SRA