Antigen

Antigen Class

TFs and others

Antigen

FOXM1

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

osteosarcoma

tissue

bone

cell line

U2OS

time point

9h

antibody

FoxM1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei were lysed and chromatin was micrococcal treated. The fragmented chromatin was pre-cleared with Dynabeads. Gamma-tubulin-, PCNA-, Mcm5-, and FoxM1-DNA-complexes were isolated with the correspondant antibody and isolated with Dynabeads. ChIP DNA using the Smarter ThruPLEX DNA-seq Kit (cat# R400676, Takara) with HT dual indexes (cat# R400660, Takara)

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

8721667

Reads aligned (%)

77.4

Duplicates removed (%)

20.1

Number of peaks

178 (qval < 1E-05)

hg19

Number of total reads

8721667

Reads aligned (%)

76.0

Duplicates removed (%)

20.7

Number of peaks

145 (qval < 1E-05)